Web18 apr. 2024 · FIGURE 1. The toxic effect of 3-HPA and 3-HP on the growth of wild type (WT) B. subtilis. Growth curves of the WT B. subtilis growing for 24 h on the M9 minimal medium with glycerol as carbon source supplemented with (A) 3-HPA (0.02–20 mM) and (B) 3-HP (1–100 mM). Both growth rate and the duration of lag phase was affected as … Web17 nov. 2014 · For BYT-glycerol medium containing no additional NaCl or NaAc, electron production by the E. coli cell membrane remained at a level of 760±35 MRU/ ... Ko and coworkers used E. coli F-122 to express HIV582-β-galactosidase fusion protein in a modified M9 minimum medium (pH 7.0) supplemented with L-tryptophan, L-leucine, L-proline and …

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WebGlycerol (10%) 100 mL: H 2 O : 800 mL: Autoclave for 20 min on liquid cycle. Cool the solution to below 60°C and add: Web13 apr. 1999 · Conditioned medium prepared from a defined M9 salts medium + 0.4% glycerol was tested for activation of the cma fusions. Weak activation was observed, ranging from 1.5- to 2-fold. However, when conditioned M9 glycerol medium was concentrated 20-fold by lyophilization and fractionated by gel filtration on a Sephadex G … logical man chords

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Web25 nov. 1997 · Cells were grown aerobically at 37°C in M9 medium (17) supplemented with 0.001% thiamine or in Luria–Bertani medium (17). Antibiotics were used at the following concentrations: ampicillin (50 μg/ml), kanamycin (50 μg/ml), and tetracycline (15 μg/ml). Bacterial growth was monitored by determining the OD at 600 nm. Bacterial Strains and … WebThree different minimal media were used: M9 minimal medium, commonly used in microbiological studies N−C− minimal medium, utilized for physiological experiments … Web8 iul. 2024 · When cells were grown in M9-glucose and M9-glycerol media, the spontaneous recombination frequency increased to about 4.3- and 7.3-fold, respectively, … industrial optimization and upgrading