WebShanghai GenePharma sirna sequence. Sirna Sequence, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 86/100, based on 1 … WebMar 20, 2024 · The Mpped1 overexpression plasmid and Pter shRNA plasmid were designed and synthesized by GenePharma ... (GenePharma, China) at a concentration of 300 μM. Briefly, 3.0 × 10 4 cells were placed into a 48-well plate (300 μl of a complete medium in each well). After the cell confluence attained 80%, the cells were co …
Did you know?
WebArticle Snippet: For RNA transfection, the cells were seeded into each well of a 24-well plate and incubated overnight and then transfected with either miR-206 mimics (Shanghai … WebBmN4 cells were cultured in TC‐100 medium (Sangon Biotech, Shanghai, China), supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher, Waltham, MA, USA), at 28 °C until they reached 50% confluency. ... being synthesized in GenePharma, Shanghai, China), (3) pmirGlO‐EndoA‐UTR and miR‐2834 mimics, and (4) pmirGlO‐EndoA‐UTR ...
WebSuzhou GenePharma is recognized world-wide as a leading provider of RNA Phosphoramidites, DNA Phosphoramidites, Special Monomers, Modifiers Reagents, … WebGenePharma Co., Ltd (NEEQ:430601) is a biotechnology company that researches and develops RNA based technologies and products headquartered in Suzhou, with …
WebApr 12, 2024 · Introduction. Rheumatoid arthritis (RA) is an autoimmune disorder that affects nearly 1% of the world’s population. 1 It is characterized by a high level of serum inflammatory cytokines, including IL-6 and TNF-α, which trigger the inflammatory process in the joints leading to progressive joint destruction and physical disability. 2, 3, 4 However, … WebThe overexpression plasmids of ASS1 and METTL14 were synthesized and cloned into pcDNA3.1 (Invitrogen, China). The short hairpin RNAs (shRNAs) targeting ASS1, METTL14, and YTHDF2 were obtained from GenePharma (Shanghai, China). ... (GenePharma, China). Lipofectamine 3000 (Invitrogen, USA) was used to transfect the …
WebSep 15, 2024 · The polymerase chain reactions (PCR) were conducted using miRcute miRNA Fluorescence Quantitation Kits (GenePharma Co. Ltd., Shanghai, China) on an Applied Biosystems 7300 (Thermo Scientific, Waltham, USA) or a FastStart Universal SYBR Green master mix (Invitrogen, CA, USA), with glyceraldehyde 3-phosphate …
WebShanghai GenePharma Co. Ltd. - Company Profiles - BCIQ. For the biopharma industry investment, business development and competitive intelligence professionals who … check audio chipset windows 10WebGenePharma (NEEQ: 430601) is a biotechnology company focusing on RNA based technologies and Pharmaceuticals. The company develops … check audio is playingWebNov 7, 2024 · Cervical cancer (CC) is one of the common gynaecological cancers and its incidence rate is the third highest among female cancers worldwide [].In China, the incidence of cervical cancer is about 96,000 … check attorney credentialsWebJun 5, 2024 · When cell growth reached 40–50% in density, cells were used for transfection using jetPRIME transfection reagent (Polyplus Transfection, France) following the manufacturer’s protocol. MiR-375 mimics and miRNA NC (negative control) were synthesized by GenePharma (Shanghai, China). check attorney recordWebMay 24, 2024 · For transfection, the cells were seeded in 24-well plates and grown to 70% to 80% confluence. The cells were transfected with miR-33a-5p mimics (or Mettl3 siRNA) using GP-transfect-Mate regent (GenePharma, Shanghai, China) according to the manufacturer’s instructions. 24 h after transfection, the cells were used for further study. check at\u0026t phone billWebJun 22, 2024 · The probes were designed and synthesized by Genepharma (Shanghai, China), and the signals of the probes were detected by a Fluorescent In Situ Hybridization Kit (Genepharma, Shanghai, China) according to the manufacturer’s instructions. The images were acquired on Lei TCS SP8 Laser Scanning Confocal Microscope (Leica … check attorney license californiaWebJan 15, 2024 · Cells were fixed with 4% paraformaldehyde for 30 min. Then, fixed cells were treated with protease reagent for 5 min, incubated with prehybridization buffer at 37 °C for 1.5 h, and hybridized with a Cy3-labelled probe (GenePharma, China) at 37 °C overnight. check attribute js